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Detailed Program
Paper Number : BI-O02
Time Frame : 14:07~14:19
Presentation Date : Thurse day, 27, November
Session Name : Biomaterials
Session Chair 1# : Byong Taek Lee
Session Chair 2# : Il Young Kim
Behaviour of Human Stem Cell on HA-TCP Composites
Indu BAJPAI
Yeungnam University
Calcium phosphate ceramics are well accepted as bone substitutes due to their excellent biocompatibility. The most widely used calcium phosphate bioceramics are hydroxyapatite [HA, Ca10(PO4)6(OH)2] and tricalcium phosphate [TCP, Ca3(PO4)2]. HA is stable in a body fluid and has been used for many years for hard tissue replacement. But, TCP which has two types (¥á and ¥â) is rather soluble compared to the HA in a body fluid. Therefore, ¥á- or ¥â-TCP materials are not used alone. The biphasic calcium phosphates (BCP) in combination with the various ratio of HA/TCP are commonly used as an artificial bone substitutes to control the biodegradation rate in a body fluid.
The aim of current study is to investigate the behavior of stem cells on HA-TCP samples. For this purpose hydroxyapatite (HA), dicalcium phosphate dehydrate (DCPD) and calcium hydroxide (Ca(OH)2) in the varying composition were mixed, cold compacted and then sintered at 1250 ¡ÆC in air environment. Quantification of HA and TCP phases was done by XRD, and a surface profilometer was used to measure the surface roughness. Wettability and surface energy of all compositions were measured by a contact angle goniometer. Cell adhesion at HA-TCP surface after incubation for two days was observed by scanning electron microscopy and fluorescence microscopy. Number of cells per unit area and area covered by cells was analyzed by the image J software. Stem cell viability and proliferation of 8 days incubated cells was observed by colorimetric (MTT) assay. The maximum viability was found at HA100D100 sample with the composition equivalent of HA-TCP (60:40) composition. The cell viability was related to the TCP content in BCP disc samples. It is believed that the TCP content above a certain HA-TCP ratio (for example, 60:40) rather reduce the stem cells viability due the fast degradation of TCP, as shown in Fig. 1
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