| Tissue engineered regeneration of completely transected femur using modified hydroxyapatite porous scaffold |
| Thuy Ba Linh NGUYEN |
| Soonchunhyang University |
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In this study, surface of porous Hydroxyapatite (HAp) scaffolds have been modified by collagen treatment and rhBMP-2 conjugation for bone regeneration. The in vitro studies were investigated to evaluate the effective of the modified surface compared to unmodified HAp surface. The rabbit model was prepared by complete removal of a 5-mm length of femur. The aim of the current study was to advance a treatment strategy for regeneration of completely transaction model, a finding that could have significant implantation for clinical application using three-dimension scaffolds HAp and HAp with collagen and BMP-2 surface modification.
Figure 1a & 1b showed an optical image and micro-CT of the HAp scaffold. The outer shell is dense, which gives ideal structural support to the scaffold jointly with the innermost thin shell. Figure 1c & d shows a surface and cross-sectional SEM micrograph of the HAp scaffolds. The fibril formation for collagen was observed by SEM on the surface and fracture surface of HAp-Collagen scaffolds, as shown in Fig.1f.
Figure 1. Photograph (a), (b) micro-CT image of HAp, (c) SEM images of surface HAp, (d) cross-section HAp; (e) Collagen coated HAp and (f) enlarge in (e).
References
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| Acknowledgements : This work was supported by Mid-career Research Program (NRF) grant number: 2009-0092808, Republic of Korea |
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