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Detailed Program
Paper Number : BI-O09
Time Frame : 16:01~16:13
Presentation Date : Thurse day, 27, November
Session Name : Biomaterials
Session Chair 1# : H. S. Yoon
Session Chair 2# : K. Uetsuki
Response of human osteoblast-like cells to different surface modified titanium alloys
J. M. Zhao
Gyeongsang National University
Titanium as one kind of biomaterials come in direct contact with the body, making evaluation of biocompatibility an important aspect to biomaterials development. While implants were traditionally allowed to “osseointegrate” over several months without carrying load, clinical and experimental data show that prostheses with roughened surfaces allow successful integration when subject to earlier loading and more challenging implant sites. However, to design implant surfaces for an optimal biological response requires an understanding of the mechanism by which roughened surfaces promote osseointegration.
So our research focus primarily on the response of osteoblastic cells to surface topography in vitro. Our in vitro test used human osteoblast cell MG-63. Cell culture experiments were conducted on surface textures. Titanium samples were chemical treated to produce morphologically similar surfaces with different roughness. Osteoblast attachment and cell viability were conducted by MTT assay and Alamar Blue assay over a different period. In addition, scanning electric microscopy was used to examine cell morphology and cell attachment on each surface, and to visualize specific interactions between cellular structures. The aim of this study was to develop Ti surface modification and to evaluate cell adhesion, proliferation, morphology on modified implant surfaces. NaOH with heat treatment of the samples increased the surface energy and improved its bioactivity further. Also, improved the bioactivity compared to other surface modification.


Figure 1 Cell viability after 1day, 2days & 3days culturing.

References:

[1] W. Suchanek and M. Yoshimura, J Mater Res, 13 (1998), p. 94–117.
[2] Morra M, Cassinelli C, Bruzzone G, Carpi A, Di Santi G and Giardino R, Int J Oral Maxillofac Implants 18(1), (2003) p. 40–5.

Acknowledgements :